diff --git a/erpnext/manufacturing/doctype/manufacturing_settings/manufacturing_settings.json b/erpnext/manufacturing/doctype/manufacturing_settings/manufacturing_settings.json
index 8d1f36ee863..1ee5bd5880a 100644
--- a/erpnext/manufacturing/doctype/manufacturing_settings/manufacturing_settings.json
+++ b/erpnext/manufacturing/doctype/manufacturing_settings/manufacturing_settings.json
@@ -15,7 +15,6 @@
   "bom_section",
   "update_bom_costs_automatically",
   "column_break_lhyt",
-  "manufacture_sub_assembly_in_operation",
   "section_break_6",
   "default_wip_warehouse",
   "default_fg_warehouse",
@@ -223,13 +222,6 @@
    "fieldname": "column_break_lhyt",
    "fieldtype": "Column Break"
   },
-  {
-   "default": "0",
-   "description": "If enabled then system will manufacture Sub-assembly against the Job Card (operation).",
-   "fieldname": "manufacture_sub_assembly_in_operation",
-   "fieldtype": "Check",
-   "label": "Manufacture Sub-assembly in Operation"
-  },
   {
    "default": "0",
    "depends_on": "eval:doc.backflush_raw_materials_based_on == \"BOM\"",
@@ -249,7 +241,7 @@
  "index_web_pages_for_search": 1,
  "issingle": 1,
  "links": [],
- "modified": "2025-01-13 12:07:03.089977",
+ "modified": "2025-02-05 16:11:11.639916",
  "modified_by": "Administrator",
  "module": "Manufacturing",
  "name": "Manufacturing Settings",
diff --git a/erpnext/manufacturing/doctype/manufacturing_settings/manufacturing_settings.py b/erpnext/manufacturing/doctype/manufacturing_settings/manufacturing_settings.py
index 04aebf23743..86b84b23339 100644
--- a/erpnext/manufacturing/doctype/manufacturing_settings/manufacturing_settings.py
+++ b/erpnext/manufacturing/doctype/manufacturing_settings/manufacturing_settings.py
@@ -29,7 +29,6 @@ class ManufacturingSettings(Document):
 		get_rm_cost_from_consumption_entry: DF.Check
 		job_card_excess_transfer: DF.Check
 		make_serial_no_batch_from_work_order: DF.Check
-		manufacture_sub_assembly_in_operation: DF.Check
 		material_consumption: DF.Check
 		mins_between_operations: DF.Int
 		overproduction_percentage_for_sales_order: DF.Percent